Method and device for measuring a target substance in a liquid sample
AssigneeMochida Pharmaceutical Co., Ltd.
InventorsTomoaki Katamine, Hiroshi Sato, Ei Mochida
Life of this patent
Estimating the term…
Abstract
A method for measuring target substance contained in a liquid sample is disclosed. The measurement is carried out by using a device having at least one reaction area comprising a solid phase, and the liquid sample is applied onto the reaction area through a porous member to allow for a reaction of predetermined volume of the liquid sample on the reaction area. A device used for such measurement is also disclosed. A device comprises a body having at least one reaction area comprising a solid phase, and a porous member disposed on or above the reaction area. The porous member allows for the liquid sample to be permeated therethrough.
Claims23
A device for measuring a target substance in a liquid sample comprising:
- a first hollow tubular member;
- a liquid-impermeable plastic disk arranged on said first member, said disk having an upper surface with a reaction area having an antibody or a fragment thereof which is reactive with said target substance on the upper surface of said disk;
- a second hollow tubular member, removably attachable to said first member, said second member having a liquid porous portion located above the reaction area of said first member when said second member is attached to said first member;
- and
- a boundary member which surrounds an edge of the reaction area in order to create a space between said upper surface of said disk and said lower surface of said second member, wherein said space the liquid sample volume is restricted to comprises a predetermined volume of not more than 5 .mu.l.
A device for measuring a target substance in a liquid sample comprising:
- a body having at least one reaction area on a substantially liquid impermeable sheet and an immobilized substance on said reaction area, which is reactive with the target substance in the liquid sample, and
- a porous member having at least one porous portion which is permeable to the liquid sample, said porous being disposed above said reaction area so as to contain a predetermined volume of not more than 5 .mu.l of said liquid sample on said reaction area, wherein said immobilized substance is an antibody or a fragment thereof which is reactive with a tumor-associated antigen.
A device for measuring a target substance in a liquid sample comprising:
- a body having
- at least one reaction area on a substantially liquid impermeable sheet;
- an immobilized substance on said reaction area, which is reactive with the target substance in the liquid sample;
- a base having a cylindrical form, said porous member being attached to the upper surface of said base;
- and
- a spacer which is attached to the upper surface of said liquid-impermeable sheet;
- and
- a porous member having at least one porous portion which is permeable to the liquid sample, said porous portion being disposed above said reaction area so as to contain a predetermined volume of not more than 5 .mu.l of said liquid sample on said reaction area, wherein said immobilized substance is an antibody or a fragment thereof which is reactive with a tumor-associated antigen.
A method for measuring a target substance in a liquid sample by means of a device having:
- a body having at least one reaction area on a substantially liquid impermeable sheet and an immobilized substance on said reaction area, which is reactive to the target substance in the liquid sample;
- and
- a porous member having at least one porous portion which is permeable to the liquid sample, said porous being disposed above said reaction area so as to contain a predetermined volume of not more than 5 .mu.l of said liquid sample on said reaction area wherein said immobilized substance comprises an antibody or fragment thereof which is reactive with a tumor-associated antigen;
- comprising the steps of:
- supplying said liquid sample through said porous portion onto said reaction area;
- removing the excess volume of the liquid sample by contacting a remover with the excess volume of the liquid sample on said upper surface of said porous portion;
- allowing said target substance to react with said immobilized substance;
- rinsing unbound liquid from said reaction area;
- removing said porous member form the device;
- and
- measuring said target substance on said reaction area.
A method for measuring a target substance in a liquid sample by means of a device comprising:
- a body having
- at least one reaction area on a substantially liquid impermeable sheet;
- an immobilized substance on said reaction area, which is reactive with the target substance in the liquid sample;
- a base having a cylindrical form, said porous member being attached to the upper surface of said base;
- a spacer which is attached to the upper surface of said liquid-impermeable sheet;
- and
- a porous member having at least one porous portion which is permeable to the liquid sample, said porous portion being disposed above said reaction area so as to contain a predetermined volume of not more than 5 .mu.l of said liquid sample on said reaction area, wherein said immobilized substance comprises an antibody or a fragment thereof which is reactive with a tumor-associated antigen;
- comprising the steps of:
- supplying said liquid sample through said porous portion onto said reaction area;
- removing the excess volume of the liquid sample by contacting a remover with the excess volume of the liquid sample on said upper surface of said porous portion;
- allowing said target substance to react with said immobilized substance;
- rinsing unbound liquid from said reaction area;
- removing said porous member from the device;
- and
- measuring said target substance on said reaction area.
Dependencies are inferred from the claim text.