DNA sequences encoding bVEGF120 and hVEGF121 and methods for the production of bovine and human vascular endothelial cell growth factors, bVEGF.sub.120 and hVEGF.sub.121
AssigneeScios Nova Inc.
InventorsEdmund G. Tischer, Judith A. Abraham, John C. Fiddes, Richard L. Mitchell
Life of this patent
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Abstract
Isolated DNA sequences, expression vectors and transformant cells are provided which allow for the large scale production of vascular endothelial cell growth factor. The vascular endothelial cell growth factor is useful in the treatment of wounds in which neovascularization or reendothelialization is required for healing.
Claims8
An isolated DNA sequence which encodes bovine vascular endothelial cell growth factor, bVEGF.sub.120, consisting essentially of the amino acid sequence of Ala at position 1 to Arg at position 120 of FIG. 6.
An isolated DNA sequence which encodes human vascular endothelial cell growth factor, hVEGF.sub.121, consisting essentially of the amino acid sequence of Ala at position 1 to Arg at position 121 of FIG. 7.
An expression vector comprising a DNA sequence encoding bVEGF.sub.120, consisting essentially of the amino acid sequence of Ala at position 1 to Arg at position 120 of FIG. 6, operably linked to a regulatory sequence capable of directing expression of the DNA sequence in a host cell.
An expression vector comprising a DNA sequence encoding hVEGF.sub.121, consisting essentially of the amino acid sequence of Ala at position 1 to Arg at position 121 of FIG. 7, operably linked to a regulatory sequence capable of directing expression of the DNA sequence in a host cell.
A method of producing vascular endothelial cell growth factor which comprises:
- culturing mammalian cells transformed with an expression vector containing a DNA sequence consisting essentially of a DNA sequence which encodes the amino acid sequence encoded by exons I-V and VIII of FIG. 8 under conditions in which the encoded polypeptide is expressed and mature hVEGF.sub.121 is secreted from the cells;
- and recovering the mature hVEGF.sub.121 from the culture medium.
Dependencies are inferred from the claim text.