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US 5,217,889 AGranted
Methods and applications for efficient genetic suppressor elements
OwnerIgor B. Roninson, Tatyana Holzmayer +1inventors
InventorsIgor B. Roninson, Tatyana Holzmayer, Kyunghee Choi
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Abstract
Methods for isolating and identifying genetic elements that are capable of inhibiting gene function are disclosed, as well as genetic elements isolated or identified according to the method of the invention and host cells modified by genetic modification using genetic suppressor elements according to the invention.
Claims3
A method of obtaining genetic suppressor elements comprising the steps of:
- (a) randomly fragmenting DNA homologous to the gene to be suppressed, to yield DNA fragments of about 700 base pair or less;
- (b) transferring the DNA fragments to expression vectors to yield a library, wherein the expression vectors are capable of expressing the DNA fragments in a living cell in which gene suppression can be selected or screened;
- (c) genetically modifying living cells by introducing the genetic suppressor element library into the living cells;
- (d) isolating or enriching for genetically modified living cells containing genetic suppressor elements by selecting or screening for gene suppression, and;
- (e) obtaining the genetic suppressor element from the genetically modified cells.
2 more elements
A method of obtaining living cells containing genetic suppressor elements comprising the steps of:
- (a) genetically modifying the living cells by introducing a library comprising randomly fragmented DNA sequences of about 700 base pairs or less;
- wherein the DNA sequences are homologous to a portion of the gene to be suppressed, and wherein the library is capable of expressing the DNA sequences in the living cell, and;
- (b) isolating or enriching for genetically modified living cells containing genetic suppressor elements by screening or selecting for gene suppression.
Dependencies are inferred from the claim text.