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AssigneeWashington University
InventorsHenry V. Huang, Robin Levis, Charles M. Rice, Sondra Schlesinger, Ping Shen, Cheng Xiong
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Compositions and methods are disclosed for expressing heterologous coding sequences in host cells. The compositions include infectious Sindbis virus RNA molecules containing at least one heterologous coding sequence inserted within the structural region of the Sindbis virus genome. RNA molecules consisting essentially of a Sindbis virus junction region are also provided. Methods utilizing the novel compositions of the present invention to express heterologous coding sequences in transformed host cells are provided. Methods for producing infectious Sindbis virus particles containing infectious Sindbis virus RNA molecules comprising at least one heterologous coding sequence are provided. This invention was made with government support under grant numbers AI24134, AI11377 and AG05681 awarded by the National Institutes of Health. The government has certain rights in the invention.
A self-replicating and self-packaging recombinant Sindbis virus RNA molecule which comprises a heterologous coding sequence located upstream from the virus structural coding region and at least five nucleotides downstream from the start of the Sindbis virus subgenomic RNA, and a Sindbis virus junction region located upstream form the virus structural coding region and downstream form the heterologous coding sequence.
A self-replicating and self-packaging recombinant Sindbis virus RNA molecule which comprises a heterologous coding sequence located downstream from the virus structural coding region and upstream from the first about 19 nucleotides immediately adjacent to and upstream from the viral polyA sequence.
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