Fluorescence measurement apparatus with an automatic recorder full-scale setting function
AssigneeShimadzu Corporation
InventorTsutomu Hayashi
Life of this patent
Estimating the term…
Abstract
A fluorescence measurement apparatus for automatically setting the full scale of the recorder in recording the spectrum curve of the fluorescence measurement. The controller of the fluorescence measurement apparatus operates as follows: a) detects peaks of the spectrum curve, b) detects the maximum value of the peaks disregarding peaks due to a cause or causes other than the fluorescent light of the sample, and c) sets the full scale of the recorder using the detected maximum value. Operations of the automatic full scale setting are described for both the excitation spectrum measurement mode and the emission spectrum measurement mode.
Claims18
A fluorescence measurement apparatus for analyzing a spectrum curve of fluorescent light emitted from a sample irradiated by an excitation light, comprising:
- an excitation light generator;
- an emitted light detector;
- scanning means for holding one of the emitted light detector and the excitation light generator at a fixed wavelength and scanning the other of the emitted light detector and excitation light generator over a range of wavelengths;
- a memory for storing data of the spectrum curve;
- and
- analyzing means for analyzing the stored data to determine data corresponding to peaks of the spectrum curve about multiples of the fixed wavelength and data corresponding to minima of the spectrum curve adjacent the determined peaks, and for generating a corrected spectrum curve by disregarding data between the determined minima corresponding to the determined peaks;
- scale setting means for detecting a maximum peak value of the corrected spectrum curve and setting a scale based on the detected maximum peak value;
- and
- a recorder for recording the corrected spectrum curve, wherein a full scale of the recorder is set to the scale set by the scale setting means.
A fluorescence measurement apparatus for analyzing a spectrum curve of fluorescent light emitted from a sample irradiated by an excitation light, comprising:
- an excitation light generator;
- an emitted light detector;
- scanning means for holding one of the emitted light detector and the excitation light generator at a fixed wavelength and scanning the other of the emitted light detector and excitation light generator over a range of wavelengths;
- a memory for storing data of the spectrum curve;
- and
- analyzing means for analyzing the stored data to determine data corresponding to peaks of the spectrum curve about multiples of the fixed wavelength and data corresponding to minima of the spectrum curve adjacent the determined peaks, and for generating a corrected spectrum curve by removing from the memory data between the determined minima corresponding to the determined peaks;
- scale setting means for detecting a maximum peak value of the corrected spectrum curve and setting a scale based on the detected maximum peak value;
- and
- a recorder for recording the corrected spectrum curve, wherein a full scale of the recorder is set to the scale set by the scale setting means.
A fluorescence measurement apparatus for analyzing a spectrum curve of fluorescent light emitted from a sample irradiated by an excitation light, comprising:
- an excitation light generator;
- an emitted light detector;
- scanning means for holding one of the emitted light detector and the excitation light generator at a fixed wavelength and scanning the other of the emitted light detector and excitation light generator over a range of wavelengths;
- a memory for storing data of the spectrum curve;
- and
- analyzing means for analyzing the stored data to determine data corresponding to peaks of the spectrum curve about multiples of the fixed wavelength and data corresponding to minima of the spectrum curve adjacent the determined peaks, and for generating a corrected spectrum curve by marking the data between the determined minima corresponding to the determined peaks;
- scale setting means for detecting a maximum peak value of unmarked data of the corrected spectrum curve and setting a scale based on the detected maximum peak value;
- and
- a recorder for recording the corrected spectrum curve, wherein a full scale of the recorder is set to the scale set by the scale setting means.
Dependencies are inferred from the claim text.