Glyceryl agarose and borate compositions
AssigneeFMC Corporation
InventorJoshua D. Smith
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Abstract
Aqueous gel compositions and their use in electrophoresis, and anhydrous compositions useful in the preparation of the aqueous gel compositions. The aqueous gel compositions comprise: A. water, B. at least one di- or tri-hydroxy C.sub.2-4 alkyl ether moiety (preferably glyceryl) substituted agarose, and C. from about 20 to about 400 mM of at least one borate compound per liter of aqueous gel composition. The anhydrous compositions comprise the agarose in powder form and the borate in powder or aqueous solution.
Claims27
An aqueous gel composition characterized in that it comprises:
- (A) at least one di-or tri-hydroxy alkyl ether moiety substituted gel-forming agarose present in a gel-forming effective amount;
- (B) at least one borate compound other than a strongly reducing or strongly axoidizing compound present in a gel-handling-effective amount;
- and
- (C) water sufficient to form a gel.
A method for gel electrophoresis characterized in that the gel used therein is an aqueous gel composition comprising:
- (A) at least one di- or tri-hydroxy C.sub.2-4 alkyl ether moiety substituted agarose present in a gel-forming-effective amount;
- (B) at least one borate compound present in a gel-handling-effective amount;
- and
- (C) water sufficient to form a gel.
A method for imparting gel-handling properties to an aqueous glyceryl agarose gel characterized by admixing therewith a handling-effective amount of at least one borate prior to, during, or subsequent to, the formation of said gel.
Dependencies are inferred from the claim text.