Pentapeptide synthesis
AssigneeImmunetech Pharmaceuticals
InventorsJohn C. Tolle, Wenying Z. Gifford, Kenneth W. Funk
Life of this patent
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Abstract
A method of synthesizing commercial quantities of the pentapeptide L-aspartyl-L-seryl-L-aspartyl-L-prolyl-L-arginine, and salts or solvates thereof, e.g., the hydrochloride or acetate salt or water solvate, compounds useful in pharmaceutical compositions for the treatment of allergic conditions, is disclosed, as are novel intermediate compounds produced in the course of the synthesis.
Claims11
A method of synthesizing L-aspartyl-L-seryl-L-aspartyl-L-prolyl-L-arginine which comprises the steps of:
- A. converting L-aspartic acid having a protected amino group and a protected side chain carboxyl group to a carboxyl-activated derivative thereof;
- B. reacting the carboxyl-activated L-aspartic acid derivative with L-proline to give an L-aspartyl-L-proline intermediate whose L-aspartyl moiety has its amino group and side chain carboxyl group protected;
- C. converting the L-aspartyl-L-proline intermediate to a carboxyl-activated derivative thereof whose L-aspartyl moiety has its amino group and side chain carboxyl group protected;
- D. reacting the carboxyl-activated L-aspartyl-L-proline intermediate with L-arginine, L-nitroarginine, or an L-arginine side chain acid salt to give an L-aspartyl-L-prolyl-L-arginine intermediate whose L-aspartyl moiety has its amino group and side chain carboxyl group protected and whose L-arginine moiety has its terminal amino group unprotected or protected as the nitroarginine derivative;
- E. deprotecting the L-aspartyl-L-prolyl-L-arginine intermediate to give L-aspartyl-L-prolyl-L-arginine;
- F. converting L-serine having a protected amino group and an unprotected or protected side chain hydroxyl group to a carboxyl-activated derivative thereof;
- G. reacting the carboxyl-activated L-serine derivative with L-aspartyl-L-prolyl-L-arginine to give an L-seryl-L-aspartyl-L-prolyl-L-arginine intermediate whose L-seryl moiety has its amino group protected and side chain hydroxyl group protected or unprotected;
- H. deprotecting the amino group in the L-seryl moiety of the L-seryl-L-aspartyl-L-prolyl-L-arginine intermediate;
- I. reacting the deprotected amino group-containing L-seryl-L-aspartyl-L-prolyl-L-arginine with a carboxyl-activated derivative of L-aspartic acid, the L-aspartic acid having a protected amino group and a protected side chain carboxyl group, to give an L-aspartyl-L-seryl-L-aspartyl-L-prolyl-L-arginine intermediate whose terminal L-aspartyl moiety has its amino group and side chain carboxyl group protected and whose L-seryl moiety has its side chain hydroxyl group protected or unprotected;
- J. deprotecting the L-aspartyl-L-seryl-L-aspartyl-L-prolyl-L-arginine intermediate to give L-aspartyl-L-seryl-L-aspartyl-L-prolyl-L-arginine.
A method which comprises reacting an L-seryl-L-aspartyl-L-prolyl-L-arginine, whose L-seryl moiety has its side chain hydroxyl group protected, with a carboxyl-activated L-aspartic acid derivative having a protected amino group and a protected side chain carboxyl group to give L-aspartyl-L-seryl-L-aspartyl-L-prolyl-L-arginine whose terminal L-aspartyl moiety has its amino group and its side chain carboxyl group protected and whose L-seryl group has its side chain hydroxyl group protected.
A method which comprises reacting O-benzyl-L-seryl-L-aspartyl-L-prolyl-L-arginine hydrochloride with N-carbobenzoxy-.beta.-benzyl-L-aspartic acid p-nitrophenyl ester to give N-carbobenzoxy-O-benzyl-L-aspartyl-O-benzyl-L-seryl-L-aspartyl-L-prolyl-L- arginine.
A method which comprises reacting L-seryl-L-aspartyl-L-prolyl-L-arginine with a carboxyl-activated L-aspartic acid derivative having a protected amino group and a protected side chain carboxyl group to give L-aspartyl-L-seryl-L-aspartyl-L-prolyl-L-arginine whose terminal L-aspartyl moiety has its amino group and its side chain carboxyl group protected.
A method which comprises reacting L-seryl-L-aspartyl-L-prolyl-L-arginine hydrochloride with N-carbobenzoxy-.beta.-benzyl-L-aspartic acid p-nitrophenyl ester to give N-carbobenzoxy-O-benzyl-L-aspartyl-L-seryl-L-aspartyl-L-prolyl-L-arginine.
Dependencies are inferred from the claim text.