US 5,212,097 AGranted

Method for analysis of catecholamine

AssigneeHitachi, Ltd.

InventorsMasao Kamahori, Mamoru Taki, Junkichi Miura, Taro Nogami, Yoshio Watanabe

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Abstract

Catecholamines in a living body fluid are analyzed by a process which involves reacting a living body fluid sample with a boron compound of the formula: ##STR1## wherein R is hydroxyl, aryl or alkyl, to form a complex of the boron compound with a catecholamine; deproteinizing the reaction solution containing the complex; decomposing the complex to release the catecholamine; labeling the released catecholamine to form a labeled catecholamine; eluting the labeled catecholamine adsorbed on a separation column; and detecting the eluted, labeled catecholamine. Advantageously, this process enables catecholamines to be measured with a high recovery ratio and high accuracy by forming the catecholamines into a complex with a boron compound.

Claims6

Dependent claims

A method for analyzing catecholamines in a living body fluid, which comprises the steps of:

(1) reacting prior to deprofeinization a living body fluid sample with a boron compound of the formula, ##STR6## wherein R is hydroxyl, phenyl, methyl or ethyl to form a reaction solution which includes complexes of the boron compound with catecholamines,
(2) deproteinizing the reaction solution containing the complexes,
(3) decomposing the complexes to release the catecholamines,
(4) labeling the released catecholamines to form labeled catecholamines,
(5) allowing the labeled catecholamines to adsorb on a precolumn packed with a cation exchange resin and removing impurities by passing an appropriate pretreatment liquid through the precolumn,
(6) eluting the labeled catecholamines adsorbed on the precolumn,
(7) passing the eluting liquid including labeled catecholamines to a separation column
(8) eluting the separate labeled catecholamines,
(9) detecting the eluted, labeled catecholamines.

6 more elements

Dependencies are inferred from the claim text.

Facts

Application: 07/616,246Filed 20 Nov 1990
Priority: JP 1-30218722 Nov 1989
Granted: 18 May 1993
Term ends: Not estimatedThe source records don’t support an estimate.

Classification12 codes

G01N 33/58
Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing:…
G01N 33/9453
Involving narcotics or drugs or pharmaceuticals, neurotransmitters or associated receptors: cardioregulators, e.g. antihypotensives, antiarrhythmics
G01N 30/24
Preparation or injection of sample to be analysed: automatic injection systems
G01N 30/54
Physical parameters: temperature