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US 5,212,096 AGranted
Gas chromatography/mass spectrometry determination of ascorbate and oxidation products thereof
AssigneeUniversity of Colorado Foundation, Inc.
InventorsJ. Fred Kolhouse, John C. Deutsch
Life of this patent
Estimating the term…
Abstract
A method for quantifying in vivo ascorbate concentration in body fluids uses gas chromatography/mass spectrometry (GC/MS). Further provided is a method for measuring redox potentials of body fluid by determining the ratio of in vivo concentration of ascorbate to one or more of its metabolites.
Claims19
A method for determination of in vivo ascorbate concentration in a body fluid comprising the steps of:
- (a) combining a known amount of ascorbate internal standard with a body fluid collected in vitro, said body fluid comprising endogenous ascorbate;
- (b) at least partially purifying said endogenous and internal standard ascorbate from other components in said in vitro body fluid;
- (c) quantitating said endogenous and internal standard ascorbate concentrations in said purified in vitro body fluid of step (b) by gas chromatography/mass spectrometry analysis;
- and
- (d) determining the in vivo ascorbate concentration by correcting the quantitated in vitro endogenous ascorbate concentration for endogenous ascorbate loss as reflected by the loss in said known amount of internal standard.
2 more elements
A method for determining the in vivo body fluid concentrations of ascorbate and metabolites thereof, comprising the steps of:
- (a) combining a known amount of an internal standard for ascorbate and each of said metabolites to be quantitated with body fluid collected in vitro, said body fluid comprising endogenous ascorbate and endogenous ascorbate metabolites;
- (b) at least partially purifying said endogenous ascorbate, endogenous ascorbate metabolites, ascorbate internal standard and metabolite internal standards in said purified body fluid of step (a) from other components in said body fluid;
- (c) quantitating the concentrations of said endogenous ascorbate, endogenous metabolites, ascorbate internal standard and metabolite internal standard by gas chromatography/mass spectrometry analysis;
- and
- (d) determining the in vivo concentration of ascorbate and metabolites by correcting the quantitated in vitro concentrations of endogenous ascorbate and endogenous metabolites for the endogenous ascorbate and metabolites loss as reflected by the loss of ascorbate and metabolite internal standards, respectively.
2 more elements
Dependencies are inferred from the claim text.