Sequence for stabilizing proteins in bacteria
AssigneeBoard of Regents, The University of Texas System
InventorWilliam G. Haldenwang
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Abstract
The invention relates to a protein stabilizing sequence particularly useful for stabilization of proteolytically sensitive proteins. The sequence includes a relatively small number of amino acids that may be expressed fused with a proteolytically sensitive protein. The most effective stabilization sequences assume .alpha.-helix structures with a hydrophobic face and a positively charged polar face which appear to require proper orientation with respect to each other. Other aspects of the invention include cloning vectors incorporating a gene sequence encoding the stabilization polypeptide and production of stabilized antigenic proteins.
Claims9
An isolated and purified peptide consisting essentially of about 29-31 amino acids contained in an inactive precursor protein of sporulation specific Bacillus subtilis factor, wherein said peptide is capable of enhancing protein stability when attached to a protein sensitive to degradation.
An isolated and purified peptide consisting essentially of 30 amino acid residues in accordance with SEQ ID NO:1 wherein arginine is inserted at position 10 between the his and leu residues.
An isolated and purified peptide consisting essentially of 32 amino acid residues in accordance with SEQ ID NO:
- 1 by insertion of arginine, leucine and alanine between the amino acid residues at positions 10 and 11.
Dependencies are inferred from the claim text.