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US 5,212,080 AGranted
Method of DNA sequencing using DNA transposon Tn5seql
AssigneeWashington University
InventorsDilip K. Nag, Henry V. Huang, Douglas E. Berg
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Abstract
A novel transposon useful for sequencing long DNAs is disclosed which comprises a partial sequence of transposon Tn5 with the oligonucleotide primers from phages SP6 and T7 inserted near the opposite ends, respectively, of said transposon Tn5.
Claims1
A method of bidirectional DNA sequencing comprising inserting into a DNA molecule of an E. coli cell a transposon Tn5seq1 having the nucleotide sequence shown in FIGS. 4A and 4B.
Dependencies are inferred from the claim text.