Process for the preparation of ascorbic acid-2-phosphate
AssigneeKyowa Hakko Kogyo Co., Ltd.
InventorsTatsuro Fujio, Akihiko Maruyama, Satoshi Koizumi
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Abstract
A process for the preparation of ascorbic acid-2-phosphate, which comprises reacting ascorbic acid or araboascorbic acid with a phosphate donor other than ATP in an aqueous medium in the presence of an effective amount of an enzyme derived and capable of preferentially catalyzing the enzymatic phosphorylation in the 2-position of ascorbic acid or araboascorbic acid by said phosphate donor, and recovering the resultant ascorbic acid-2-phosphate from the reaction solution.
Claims8
A process for the preparation of ascorbic acid-2-phosphate, which comprises the steps of reacting ascorbic acid with a phosphate donor other than ATP in an aqueous medium in the presence of an effective amount of an enzyme derived from a culture of a microorganism capable of preferentially catalyzing the enzymatic phosphorylation at the 2-position of said ascorbic acid by said phosphate donor, and then recovering the resultant ascorbic acid-2-phosphate from the reaction solution, wherein said microorganism is chosen form the group consisting of
- Klebsiella oxytoca, ATCC 8724;
- Cellumonas cartai, ATCC 21681;
- Alcaligenes eutrophus, ATCC 17697
- Beneckea hyperoptica, ATCC 15803;
- Aeromonas hydrophila, ATCC 11163;
- Klebsiella pneumoniae, ATCC 21524;
- Brevibacterium lyticum, ATCC 15921;
- Brevibacterium ammoniagenes, ATCC 6872;
- Brevibacterium flavum, ATCC 13826;
- Brevibacterium lactofermentum, ATCC 13655;
- Pseudomonas riboflavina, ATCC 9526;
- Pseudomonas diminuta, ATCC 11568;
- Flavobacterium devorans, ATCC 10829;
- Pseudomonas azotocolligans, ATCC 12417
- Pseudomonas maltophilia, ATCC 17806;
- Morganella morganii, ATCC 25830;
- Serratia rubidaea, ATCC 11634;
- Enterobacter aerogenes, ATCC 13048;
- and
- Corynebacterium glutamicum, ATCC 13032.
Dependencies are inferred from the claim text.