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AssigneesThe Johns Hopkins University, The Brigham and Women's Hospital, Inc., T Cell Sciences, Inc.
InventorsDouglas T. Fearon, Lloyd B. Klickstein, Winnie W. Wong, Gerald R. Carson, Michael F. Concino, Stephen H. Ip, Savvas C. Makrides
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The present invention relates to the C3b/C4b receptor (CR1) gene and its encoded protein. The invention also relates to CR1 nucleic acid sequences and fragments thereof comprising 70 nucleotides and their encoded peptides or proteins comprising 24 amino acids. The invention further provides for the expression of the CR1 protein and fragments thereof. The genes and proteins of the invention have uses in diagnosis and therapy of disorders involving complement activity, and various immune system or inflammatory disorders. In specific embodiments of the present invention detailed in the examples sections infra, the cloning, nucleotide sequence, and deduced amino acid sequence of a full-length CR1 cDNA and fragments thereof are described. The expression of the CR1 protein and fragments thereof is also described. Also described is the expression of a secreted CR1 molecule lacking a transmembrane region. The secreted CR1 molecule is shown to be useful in reducing damage caused by inflammation and in reducing myocardial infarct size and preventing reperfusion injury.
An isolated nucleic acid encoding a polypeptide, the amino acid sequence of which comprises at least a fragment of the amino acid sequence depicted in FIG. 1, which polypeptide has a complement regulatory activity.
A recombinant DNA vector comprising piABCD.
A recombinant DNA vector comprising piABCD, as deposited with the NRRL and assigned accession number B-18355.
An Escherichia coli comprising piABCD, as deposited with the NRRL and assigned accession number B-18355.
An isolated nucleic acid encoding a soluble CR1 molecule substantially lacking a transmembrane region, which CR1 molecule has a complement regulatory activity.
A recombinant DNA vector comprising pBSCR1c/pTCSgpt, as deposited with the ATCC and assigned accession number CRL 10052.
A Chinese hamster ovary cell DUX B11 carrying plasmid pBSCR1c/pTCSgpt, as deposited with the ATCC and assigned accession number CRL 10052.
An isolated nucleic acid comprising the DNA sequence depicted in FIG. 1 or a subsequence of the DNA sequence depicted in FIG. 1, which nucleic acid encodes a polypeptide or fragment thereof, which polypeptide or fragment has a complement regulatory activity.
An isolated first nucleic acid characterized as being hybridizable under conditions of high stringency that do not allow detection of CR2 cDNA clones to a second nucleic acid, which second nucleic acid comprises at least a fragment of the sequence depicted in FIG. 1, and which first nucleic acid encodes a polypeptide, which polypeptide has a complement regulatory activity.
Dependencies are inferred from the claim text.