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AssigneeBoehringer Mannheim GmbH
InventorsBeatus Ofenloch-Hahnle, Michael Berger, Arno Deger
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The present invention provides a process for the detection of analytes in body fluids containing free biotin by immunoassay with the use of biotin conjugates for the labelling, immobilization or complexing of immunological reagents, wherein the biotin present in free form is removed from the immunological reaction by incubating the sample solution with polymer particles consisting of a core and a covering which, as core, contain a polymer which has a plurality of binding sites for biotin and, as covering, at least one layer of protein, peptide, carbohydrate or co-polymer of carbohydrate and amino acids, the layer thickness of the covering being so adjusted that the coating is permeable for the free biotin but not for the biotin conjugate.
In an immunoassay for determining an analyte in a body fluid sample, wherein said sample is contacted with a conjugate of biotin and a receptor for said analyte and any complexes formed between said conjugate and analyte are determined, and wherein said sample contains in addition to said analyte, free biotin or a derivative of free biotin, the improvement comprising adding to said body fluid sample a water soluble, polymeric particle consisting of:
Ligand trap consisting essentially of:
Dependencies are inferred from the claim text.