Direct binding assay for the determination of a bacteroids organism
AssigneeEastman Kodak Company
InventorsBrian A. Snyder, Paul B. Contestable, Catherine T. Abrams, Joseph J. Zambon, Homer S. Reynolds
Life of this patent
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Abstract
Antigens extracted from one or more serotypes of a Bacteroides microorganism are rapidly and sensitively detected when directly bound to a water-insoluble substrate. The bound antigens are detected by forming an immunological complex on the substrate when the antigen reacts with the appropriate antibody. The antibody-antigen complex can be detected directly or by means of a second antibody which is directed to the Bacteroides antibody. The entire assay can be carried out at room temperature in less than about 15 minutes.
Claims20
A method for the determination of a Bacteroides microorganism comprising:
- A. contacting at least one antigen extracted from a specimen suspected of containing a Bacteroides microorganism with a microporous membrane for a sufficient time for said Bacteroides antigen to directly bind to said membrane,
- B. within about five minutes of said contacting, separating unbound materials from said Bacteroides antigen bound to said membrane by washing said unbound materials through said membrane, and contacting said bound antigen with at least one antibody of said Bacteroides antigen to form an immunological complex bound to said membrane, and
- C. within about five minutes of contacting said antibody with said bound antigen, separating said bound immunological complex from uncomplexed antibody by washing said uncomplexed antibody through said membrane, and determining the presence of said bound complex on said membrane as an indication of the presence of said Bacteroides microorganism in said specimen,
- said method being carried out within about 15 minutes.
A method for the determination of one or more serotypes of the microorganisms Bacteroides intermedius comprising:
- A. extracting at least one antigen from one or more serotypes of Bacteroides intermedius present in a biological specimen,
- B. contacting said extracted antigen with a microporous membrane for a sufficient time for said extracted antigen to directly bind to said membrane,
- C. within about two minutes of said contacting, separating unbound materials from said bound antigen by washing said unbound materials through said membrane, and contacting said bound antigen with a labeled antibody to said antigen to form a labeled immunological complex bound to said membrane,
- D. within about five minutes of said contacting in step C, separating said labeled complex bound to said membrane from uncomplexed materials by washing said uncomplexed materials through said membrane, and
- E. determining said labeled bound complex on said membrane as a measure of the amount of one or more serotypes of Bacteroides intermedius in said specimen,
- said method being carried out within about 15 minutes.
A method for the determination of one or more serotypes of the microorganism Bacteroides (Porphyromonas) gingivalis comprising:
- A. extracting at least one antigen from one or more serotypes of Bacteoides (Porphyromonas) gingivalis present in a biological specimen,
- B. contacting said extracted antigen with a microporous membrane for a sufficient time for said extracted antigen to directly bind to said membrane,
- C. within about two minutes of said contacting, separating unbound materials from said antigen bound to said membrane by washing said unbound materials through said membrane, and contacting said bound antigen with a labeled antibody to said antigen to form an immunological complex bound to said membrane,
- D. within about five minutes of said contacting in step C, separating said labeled complex bound to said membrane from uncomplexed materials by washing said uncomplexed materials through said membrane, and
- E. determining said labeled complex bound to said membrane as a measure of the amount of one or more serotypes of Bacteroides (Porphyromonas) gingivalis in said specimen,
- said method being carried out within about 15 minutes.
Dependencies are inferred from the claim text.