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US 5,211,660 AGranted
Method for performing epikeratophakia by electrofusion
AssigneeUniversity of South Florida
InventorRobert J. Grasso, deceased
Life of this patent
Estimating the term…
Abstract
An improved epikeratophakia utilizing electrofusion to graft donor corneal tissue to patient's cornea.
Claims26
Epikeratophakia comprising the steps of:
- (a) contacting donor cornea tissue having preselected topology of convex dimensions with a patient's own cornea, using mechanical force applied by a device having concave dimensions congruent to the preselected convex dimensions of said donor tissue;
- and
- (b) subjecting the donor cornea tissue and the patient's cornea to an electrical field under conditions sufficient to fuse the donor tissue to the patient's cornea.
Epikeratophakia comprising the steps of:
- (a) obtaining a donor corneal tissue suitable for grafting to patient's cornea to correct a diagnosed hyperopic or myopic defect;
- (b) forming a crevice in patient's cornea by cutting a peripheral groove therein;
- (c) obtaining monocytes from a blood source;
- (d) introducing a first portion of the monocytes into the crevice;
- (e) introducing a second portion of the monocytes in binding relationship to the periphery of the donor corneal tissue;
- (f) positioning the donor corneal tissue in congruent relationship with patient's cornea such that the first portion of monocytes is in direct contact with the second portion of monocytes;
- and
- (g) subjecting the positioned donor corneal tissue and patient's cornea to an electric field under conditions sufficient to graft the donor corneal tissue to patient's cornea.
5 more elements
Method for corneal grafting which comprises the steps of:
- (a) obtaining a hydrated shaped donor corneal tissue having a preselected dimensional shape;
- (b) preparing annular keratectomy site by trephination of a crevice on patient's cornea;
- (c) obtaining mononuclear phagocytic leukocytes from a blood source;
- (d) sensitizing the mononuclear phagocytic leukocytes by admixing with a suitable anticollagen antibody at the Fc regions;
- (e) depositing the hydrated shaped donor corneal tissue on a hemispherical shaped plate to form a peripheral reservoir about the donor tissue;
- (f) introducing a first portion of the sensitized mononuclear phagocytic leukocytes into peripheral reservoir under conditions sufficient to bind such mononuclear phagocytic leukocytes to the donor tissue;
- thereby forming a graft donor tissue;
- (g) introducing a second portion of sensitized mononuclear phagocytic leukocytes into annular keratectomy site on the patient's cornea;
- (h) positioning the graft donor tissue into the keratectomy site crevice in patient's cornea;
- and
- (i) subjecting the graft donor tissue and the patient's cornea to an electrical field while maintaining the graft donor tissue positioned into the keratectomy site crevice in patient's cornea, under conditions sufficient to graft the donor tissue to patient's cornea by fusion.
8 more elements
An improved epikeratophakia process, comprising the steps of:
- contacting a living patient's cornea with donor cornea tissue having a preselected topology of convex dimensions;
- and
- subjecting the donor cornea tissue and the patient's cornea to a direct current electrical field under conditions sufficient to fuse the donor tissue to the patient's cornea.
Dependencies are inferred from the claim text.