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US 5,210,185 AGranted
Human nerve growth factor
AssigneeFidia S.p.A.
InventorsFrancesco Della Valle, Lanfranco Callegaro, Silvana Lorenzi
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Abstract
The present invention is directed to a method for the isolation of Human Nerve Growth Factor (HNGF) from human placenta. More particularly, the invention is directed to the method of isolation of the .beta.-subunit of Human Nerve Growth Factor and Compositions containing the same for the treatment of neuropathological conditions.
Claims7
A process for preparing a biologically active .beta. subunit of human nerve growth factor from human placenta comprising the steps of:
- a) homogenizing the tissue of human placenta,
- b) centrifuging the homogenate and recovering the supernatant,
- c) dialyzing the supernatant at a pH of from 3.5 to 4.5 in order to dissociate the 7S NGF form,
- d) fractionating by chromatography the resultant solution on a three step cation exchange chromatography column, p1 e) purifying the active fraction obtained by cation exchange chromatography with a gradient of sodium chloride and pH from 5.5 to 6.5 and
- f) purifying the active fraction thus obtained by affinity chromatography on polyclonal antibodies, raised against mouse NFG.
2 more elements
Dependencies are inferred from the claim text.