Antibodies to cytokinins having a glycosylated isoprenoid side chain and immunoassay methods
AssigneeThe United States of America as represented by the Secretary of Agriculture
InventorsDavid L. Brandon, Joseph W. Corse
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Abstract
Antibodies (polyclonal and monoclonal) having specificity for cytokinins having a glycosylated isoprenoid side chain are described. The antibodies simultaneously recognize a purine ring, an isoprenoid side chain, and a 4'-O-glycoside. The antibodies were elicited using a novel hapten, 9-(2-carboxyethyl) cytokinin-O-glycoside. Immunoassay methods for the determination of cytokinins having a glycosylated isoprenoid side chain which utilize the antibodies are also described.
Claims9
Antibody which is specific for a cytokinin, said cytokinin possessing a glycosylated isoprenoid side chain, that is, it is able to simultaneously recognize a purine ring, an isoprenoid side chain, and a 4'-O-glycoside.
A compound, 9-(2-carboxyethyl) cytokinin-O-glycoside, having the formula selected from ##STR2## wherein R is glucoside, riboside, or substituted glucoside or riboside.
A composition capable of raising an immunological response in an immunologically competent animal comprising a cytokinin having a purine ring, an isoprenoid side chain, and a 4'-O-glycoside coupled to an immunogenic carrier.
Dependencies are inferred from the claim text.