Method to enhance TPA production
AssigneeCentocor, Inc.
InventorsRandall G. Rupp, John C. Lane
Life of this patent
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Abstract
A medium supplement which promotes the secretion of tPA from cells producing this protein natively or by virtue of recombinant transformation without the concomitant addition of contaminating proteins is described. Autoclaved brain/heart infusion preparations contribute virtually no extraneous proteins to the medium but greatly enhance the secretion of tPA.
Claims8
A method to improve production of tissue plasmiogen activator (tPA) in a culture of a tPA-producing cell line which tPA-producing cell line is a transformed mammalian cell line that produces tPA by virtue of a recombinant expression system,
- which method comprises culturing said transformed cell line capable of producing tPA in a basal medium which is specifically suitable for said cell line wherein said basal medium further contains an amount of a preparation of brain/heart infusion (BHI) effective to improve tPA production by said cell line.
A cell culture comprising a mammalian cell line capable of secreting tissue plasminogen activator (tPA) wherein said cell line is a transformed mammalian cell line that normally produces tPA or that produces tPA by virtue of a recombinant expression system, and
- a basal medium which is specifically suitable for said cell line and which further contains an amount of a preparation of brain-heart infusion (BHI) effective to improve tPA production by said cell line.
Dependencies are inferred from the claim text.