Pseudomonas screening assay
AssigneeThe United States of America as represented by the Administrator of the National Aeronautics and Space Administration
InventorRuth Margalit
Life of this patent
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Abstract
A screening assay for detecting the presence of Pseudomonas bacteria in a test sample comprising isolating the bacteria present in the sample, extracting Azurin protein from the periplasm within the bacteria, reacting the extract with an enzymatically labelled, Azurin-specific antibody and a color-producing substrate specific for the enzyme which is capable of producing a color distinct from the color generated by Azurin and reading the results of the reaction of the sample with the antibody to determine whether the color has been produced whereby the presence of Pseudomonas bacteria in the sample is indicated.
Claims15
A screening assay for detecting the presence of Pseudomonas bacteria in a test sample comprising isolating the bacteria present in said sample, extracting Azurin protein from periplasm within the bacteria in said sample, reacting said extract with an enzymatically labelled, Azurin-specific antibody and a color-producing substrate specific for said enzyme and capable of producing a color distinct from the color generated by Azurin and reading the results of said reaction of said sample with said antibody to determine whether said color has been produced whereby the presence of Pseudomonas bacteria in said sample is indicated.
A method for screening a body fluid for the presence of Pseudomonas bacteria utilizing Azurin as a marker for said bacteria comprising;
- obtaining a test body fluid sample from a patient;
- centrifuging said sample to isolate the bacteria therein and extracting the Azurin from said bacteria;
- immobilizing the resulting Azurin extracted from said sample on a membrane;
- contacting the membrane with an Azurin-specific, enzymatically labelled antibody solution;
- and
- reacting the antibody with a color-producing substrate specific for said enzyme and capable of developing a color distinct from that of Azurin and reading the results to determine whether Pseudomonas bacteria were present in the test sample as evidenced by the presence of said color on the membrane.
Dependencies are inferred from the claim text.