Back to results
US 5,208,350 AGranted
7-hydroxy coumarins having substitutions in the 4 position
AssigneeAbbott Laboratories
InventorsStanley R. Bouma, Joseph E. Celebuski
Life of this patent
Estimating the term…
Abstract
This invention relates to fluorescent 7-hydroxy coumarin compounds with substitutions in the 4 position having a length greater than one carbon atom. The compounds thus are related to 4-methylumbelliferone (7-hydroxy-4-methyl coumarin, or 4-MU), the detectable label used in the IM.sub.x .RTM. instrument assays (Abbott Laboratories, Abbott Park, Ill.). The substitutions in the 4 position are branched and include functional groups for coupling to biological molecules.
Claims13
A compound of the formula:
- ##STR13## wherein R.sub.1 is selected from the group consisting of --CH.sub.3, --G--OZ, --G--SZ, --G-- NHY, --OZ, --SZ, --NHY, and alkyl of the formula --G--CH.sub.3, where G represents an alkylene chain having from 1 to 12 carbon atoms, and Z represents hydrogen or a protecting group for O or S, and Y represents hydrogen or a protecting group for N;
- and
- wherein R.sub.2 is selected from the group consisting of --J--SH, --J--X, --OH, --SH, and --COOR', where J represents an alkylene chain having from 1 to 10 carbon atoms, X represents a halogen, and R' represents H or an alkyl chain having from 1 to 10 carbon atoms.
Dependencies are inferred from the claim text.