Cloning the TaqI restriction and modification genes
AssigneeNew England Biolabs, Inc.
InventorGeoffrey G. Wilson
Life of this patent
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Abstract
Methods for cloning restriction enzymes and their corresponding modification enzymes by selecting clones resistant to in vitro digestion by the restriction enzyme and subsequent screening to identify those clones containing the restriction gene.
Claims8
Isolated DNA coding for the TaqI restriction endonuclease, wherein the isolated DNA is obtainable from ATCC Accession No. 75274.
A recombinant DNA vector comprising a vector into which a DNA segment coding for the TaqI endonuclease produced by Thermus aquaticus ATCC No. 25104 has been inserted.
Isolated DNA coding for the TagI restriction endonuclease and methylase, wherein the isolated DNA is obtainable from ATCC Accession No. 75274.
Dependencies are inferred from the claim text.