D-amino acid oxidase and method for isolation thereof
OwnerKlaus Mosbach, Estera Szwajcerinventors
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Abstract
A Triqonopsis variabilis D-amino acid oxidase in substantially pure form and active against cephalosporin C is disclosed. This D-amino acid oxidase is isolated from Trigonopsis variabilis by a method which is performed in three steps, namely: (a) acidifying and heating a crude cell extract of Trigonopsis variabilis to obtain a precipitate and supernatant fraction; (b) treating said supernatant fraction obtained in step (a) with sufficient ammonium sulfate to obtain a second precipitate, said second precipitate containing the D-amino acid oxidase of claim 1; and (c) resuspending the precipitate obtained in step (b) and collecting the D-amino acid oxidase by isoelectric precipitation.
Claims5
Trigonopsis variabilis D-amino acid oxidase which is active against the substrate cephalosporin C, said D-amino acid oxidase having an isoelectric point of about 4.6, existing in its active form as a dimer, having two subunits, and having a molecular weight of about 86,000.
Dependencies are inferred from the claim text.