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US 5,208,144 AGranted
Method for detection of human DNA containing the gene encoding low density lipoprotein receptor
AssigneeThe General Hospital Corporation
InventorsJohn A. Smith, Raktima Raychowdhury, John L. Niles
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Abstract
A method for the detection of a genetic abnormality in the LDL receptor gene, by hybridization of such gene with a DNA sequence encoding gp330.
Claims1
A method for the detection of human DNA which contains the gene encoding low density lipoprotein receptor, said method comprising
- (a) isolating DNA that contains said DNA which contains the gene encoding low density lipoprotein receptor from a human;
- (b) contacting said DNA obtained in step (a) with DNA encoding gp330 under for a time and under conditions sufficient for hybridization to occur;
- and
- (c) detecting hybridization of said DNA which contains the gene encoding gp330 DNA with said low density lipoprotein receptor whereby the presence of said hybridization indicates the presence of DNA which contains the gene encoding the low density lipoprotein receptor.
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Dependencies are inferred from the claim text.