Essentially pure human parathyroid hormone
AssigneesAllelix Biopharmaceuticals Inc., Glaxo Canada Inc.
InventorDennis R. Sindrey
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Abstract
Human parathyroid hormone is provided in an ultrapure form characterized by the absence of protein contaminants detectable by capillary electrophoresis analysis. A method for obtaining such ultrapure human parathyroid hormone is also described.
Claims21
Essentially pure human parathyroid hormone, characterized by single peak migration when analyzed by capillary electrophoresis at 214 nm, and by an EC50 as determined in the UMR 106-based adenylate cyclase assay of not more than 2 nM.
A method for purifying human parathyroid hormone, which comprises the step of fractionating a partially purified human PTH preparation by reversed phase high performance liquid chromatography with a cationic ion-pairing agent.
Essentially pure human parathyroid hormone obtained by the steps of
- i) obtaining a partially purified preparation of E. coli-produced human parathyroid hormone,
- ii) fractionating the partially purified preparation by reversed phase high performance liquid chromatography with triethylamine or a salt thereof;
- and
- iii) collecting, following the chromatographic step, essentially pure human parathyroid hormone.
Dependencies are inferred from the claim text.