Vaccine against avian encephalomyelitis
AssigneeMinister of Agriculture, Fisheries & Food in Majesty's Government of Great Britain
InventorsRobin A. J. Nicholas, Denise H. Thornton, Roy D. Goddard
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Abstract
A process for preparation of an attenuated strain of avian encephalomyelitis virus ("AEV") which may be used as a component of a protective vaccine, which comprises the steps of infecting chick embryo fibroblast ("CEF") cells with AEV, culturing the AEV-infected CEF cells until the AEV has become adapted to the CEF cells and then isolating the AEV product from the CEF cells for use in the vaccine. An attenuated AEV strain produced in this way, and tests showing use as a vaccine in chickens are also described.
Claims20
A process for preparation of an attenuated strain of avian encephalomyelitis virus ("AEV") which comprises the steps of:
- a. infecting chick embryo fibroblast ("CEF") cells with AEV, then
- b. culturing the infected CEF cells until the AEV has become adapted to the CEF cells, then
- c. isolating the AEV from the cultured CEF cells.
An attenuated strain of AEV which has been subjected to the following process:
- a. 5-30 times of serial passage of the AEV through CEN cells from chicken embryos,
- b. 10-20 times of serial passage of the AEV from step a through CEF cells from chicken embryos, and
- c. isolation of the AEV from the cultured GEF cells.
Dependencies are inferred from the claim text.