Biologically active Bacillus thuringiensis isolates
AssigneeMycogen Corporation
InventorsGregory A. Bradfisch, Tracy Michaels, Jewel M. Payne
Life of this patent
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Abstract
Disclosed and claimed are Bacillus thuringiensis isolates designated B.t. PS86A1 and B.t. PS86Q3, which are active against corn rootworm larvae and the alfalfa weevil. Thus, these isolates, or mutants thereof, can be used to control such pests. Further, genes encoding novel .delta.-endotoxins can be removed from these isolates and transferred to other host microbes, or plants. Expression of the .delta.-endotoxins in such hosts results in the control of corn rootworm larvae and the alfalfa weevil.
Claims17
A process for controlling corn rootworm larvae which comprises incorporating in the soil habitat of said corn rootworm larvae a corn rootworm larvae-controlling amount of Bacillus thuringiensis PS86A1 NRRL B-18900 or PS86Q3 NRRL B-1876, and mutants thereof which retain the activity against the corn rootworm, or spores of said microbes.
A method for controlling corn rootworm larvae which comprises contacting said larvae with a corn rootworm larvae-controlling amount of a Bacillus thuringiensis selected from the group consisting of Bacillus thuringiensis PS86A1, NRRL B-18400 and Bacillus thuringiensis PS86Q3, NRRL B-18765.
A composition of matter comprising a Bacillus thuringiensis mutant prepared from parent Bacillus thuringiensis PS86A1, NRRL B-18400 or Bacillus thuringiensis PS86Q3, NRRL B-18765, in association with an inert carrier.
A composition of matter for controlling corn rootworm comprising Bacillus thuringiensis PS86A1, NRRL B-18400 and Bacillus thuringiensis PS86Q3, NRRL B-18765, and mutants thereof which retain the activity against the corn rootworm, or spores of said microbes, in association with an inert carrier.
A pesticidal composition comprising substantially intact, treated cells having prolonged pesticidal activity and greater persistence in the feeding zone when applied to the environment of a target pest, wherein said pesticide is produced by Bacillus thuringiensis PS86A1, NRRL B-18400 or Bacillus thuringiensis PS86Q3, NRRL B-18765.
A method for controlling corn rootworm larvae which comprises contacting said larvae with a corn rootworm larvae-controlling amount of a pesticidal composition comprising intact treated cells having prolonged pesticidal activity when applied to the environment of corn rootworm larvae, wherein said insecticide is produced by Bacillus thuringiensis PS86A1, NRRL B-18400 or Bacillus thuringiensis PS86Q3, NRRL B-18765.
A biologically pure culture of Bacillus thuringiensis PS86Q3 having all the identifying characteristics of deposit NRRL B-18765.
A process for controlling insect infestation of alfalfa, said infestation by an alfalfa weevil, which comprises contacting said infesting insect, or treating the environment of said infesting insect, with an alfalfa weevil-controlling amount of Bacillus thuringiensis PS86Q3, NRRL B-18765, or mutants thereof which retain the activity against the alfalfa weevil, or spores from said Bacillus thuringiensis microbe.
Dependencies are inferred from the claim text.