Isotopic tracer composition and method for making and using same
AssigneeThe Board of Regents of the University of Oklahoma
InventorStanley L. Mills
Life of this patent
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Abstract
An isotopic tracer composition and methods of making and using same for radiological evaluation of body functions and diseases in a subject and for radiological therapy. The isotopic tracer composition comprises a protein carrier agent such as fibrinogen, antibodies, enzymes or portions thereof, bound to a radioisotope which has been reduced to a lower oxidation state by stannous phosphate at a pH of greater than 7. Some suitable radioisotopes are isotopes of technetium, rhenium and ruthenium.
Claims13
A kit for preparing a reagent to be used with a radioisotope and a protein or fragment thereof thereby preparing an isotopic tracer composition, wherein the radioisotope is characterized by the ability to bind a protein or fragment thereof when reduced to a lower oxidation state consisting of:
- an effective amount of an alkalizing agent to produce a pH greater than 7 in the composition;
- and
- an effective amount of stannous phosphate to reduce a scintigraphically detectable amount of the radioisotope to a lower oxidation state thereby binding the protein or fragment thereof to the radioisotope.
A kit for preparing a reagent to be used with a radioisotope and a protein or fragment thereof thereby preparing an isotopic tracer composition, wherein the radioisotope is characterized by the ability to bind a protein or fragment thereof when reduced to a lower oxidation state, consisting of:
- an effective amount of an alkalizing agent to produce a pH greater than 7 in the composition;
- an effective amount of stannous phosphate to reduce a scintigraphically detectable amount of the radioisotope to a lower oxidation state thereby binding the protein or fragment thereof to the radioisotope;
- an effective amount of a protein or fragment thereof to provide bonding sights for the scintigraphically detectable amount of radioisotope.
A kit for preparing a reagent to be used with a radioisotope and a protein or fragment thereof thereby preparing an isotopic tracer composition, wherein the radioisotope is characterized by the ability to bind a protein or fragment thereof when reduced to a lower oxidation state consisting of:
- an effective amount of an alkalizing agent to produce a pH greater than 7 in the composition;
- and
- an effective amount of stannous phosphate to reduce a scintigraphically detectable amount of the radioisotope to a lower oxidation state thereby binding the protein or fragment thereof to the radioisotope;
- and
- a sufficient amount of chelating agent to bind to a substantial amount of the protein fragments.
A kit for preparing a reagent to be used with a radioisotope and a protein or fragment thereof thereby preparing an isotopic tracer composition, wherein the radioisotope is characterized by the ability to bind a protein or fragment thereof when reduced to a lower oxidation state consisting of:
- an effective amount of an alkalizing agent to produce a pH greater than 7 in the composition;
- and
- an effective amount of stannous phosphate to reduce a scintigraphically detectable amount of the radioisotope to a lower oxidation state thereby binding the protein or fragment thereof to the radioisotope;
- and
- an effective amount of a buffering agent to produce about a physiological pH in the isotopic tracer composition.
Dependencies are inferred from the claim text.