DNA sequence encoding active fragment of fibroblast growth factor, HBF-2
AssigneeAmerican Cyanamid Company
InventorsAndrew P. Seddon, Peter Bohlen
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Abstract
The present invention relates to novel fragments of basic fibroblast growth factor (bFGF). The mitogenic potency of one of these bFGF fragments, identified as HBF-2 is about 25-50 fold less than that of native bFGF but at least 10.sup.3 -10.sup.4 fold more active than that of previously reported synthetic fragments of bFGF. Therefore, the present invention provides the shortest fragment of bFGF that retains substantial biologic activity.
Claims3
An isolated DNA molecule consisting of a sequence that codes for a fragment of mammalian recombinant basic fibroblast growth factor, the fragment consisting of amino acids 70 to 155 of basic fibroblast growth factor.
An isolated DNA molecule consisting of a sequence that codes for a fragment of mammalian recombinant basic fibroblast growth factor, the fragment consisting of amino acids 70 to 155 of mammalian basic fibroblast growth factor, the sequence modified by replacing amino acid 78 and amino acid 96 independently with an amino acid selected from the group consisting of alanine, glycine, arginine, tryptophan, lysine, aspartic acid, glutamic acid, asparagine, glutamine, histidine, isoleucine, leucine, valine, phenylalanine, tyrosine, methionine, serine, threonine and proline.
Dependencies are inferred from the claim text.