Process for making D-aminoacylase
AssigneeNational Science Council of Republic of China
InventorsYing C. Tsai, Chyuan S. Lin, Ching P. Tseng, Yunn B. Yang
Life of this patent
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Abstract
A process for making D-aminoacylse includes adding 1% N-acetyl-DL-amino acid preferably N-acetyl-DL-methionine and N-acetyl-DL-leucine in a culturing medium incubated with bacteria selected from the strain of Alcaligenes faecalis for culturing the bacteria and for inductively promoting an enzyme reaction to produce the D-aminoacylase which is able to hydrolyze D-amino acids and unable to hydrolyze L-amino acids.
Claims1
A process for producing D-aminoacylase comprising adding 1% (weight/volume) of an N-acetyl-DL-amino acid selected from the group consisting of N-acetyl-DL-methionine and N-acetyl-DL-leucine to a culturing medium, culturing bacteria of the strain Alcaligenes faecalis DA-1 having accession number CCRC 14817 in said culturing medium, and recovering D-aminoacylase from the cultured bacteria;
- said D-aminoacylase having properties including:
- a substrate specificity capable of hydrolyzing N-acetyl-D-amino acids and unable to hydrolyze N-acetyl-L-amino acids and having a relative activity of 0.8% for the reaction of said D-aminoacylase with N-acetyl-L-methionine with respect to a relative activity of 100% for the reaction of said D-aminoacylase with N-acetyl-D-methionine;
- a molecular weight of 55,000;
- an isoelectric point of 5.35;
- and being inhibited by metallic ions of mercuric, cupric and carbonyl ions and inhibited by p-chloromercuribenzoic acid, N-ethylmaleimide and EDTA.
Dependencies are inferred from the claim text.