Method of producing cecropins by microbiological techniques
AssigneeXoma Corporation
InventorsJiunu Lai, Jar-How Lee, Yun-Long Lin, Dan Ray, Gary Wilcox
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Abstract
A polynucleotide molecule expressible in a given host comprising the sequence of the araB promoter operably linked to a gene which is heterologous to said host. The heterologous gene codes for a peptide that is biologically active. The invention also relates to a genetic construct which comprises a first genetic sequence coding for cecropin operably linked to a second genetic sequence coding for a polypeptide which is capable of suppressing the bactericidal effect of the resulting fusion protein towards an otherwise cecropin sensitive bacterium.
Claims15
A genetic construct which comprises a first genetic sequence coding for a polypeptide which is capable of suppressing the bactericidal effect of the resulting fusion protein towards an otherwise cecropin-sensitive bacterium operably linked to a second genetic sequence coding for cecropin.
A cecropin-sensitive host transformed with a vehicle expressible therein, said vehicle carrying a genetic sequence coding for cecropin operably linked to a genetic sequence coding for a polypeptide capable of suppressing the bactericidal effect of said cecropin towards said host in the resulting expression fusion product.
A method of producing cecropin by microbial techniques which comprises:
- operably linking a first genetic sequence coding for cecropin with a second genetic sequence coding for a different polypeptide to form a fused gene;
- transforming said fused gene into a cecropin susceptible host;
- culturing said transformed host, whereby a protein conjugate non-toxic to said host is produced;
- and
- subsequently recovering cecropin from said protein conjugate.
Dependencies are inferred from the claim text.