Cloning and expression of early growth regulatory protein genes
AssigneeArch Development Corporation
InventorVikas P. Sukhatme
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Abstract
Disclosed are DNA sequences encoding novel DNA binding proteins implicated in regulation of early stages of cell growth. Illustratively provided are human and mouse origin DNA sequences encoding early growth regulatory ("Egr") proteins which include "zinc finger" regions of the type involved in DNA binding. Also disclosed are immunological methods and materials for detection of Egr proteins and hybridization methods and materials for detection and quantification of Egr protein related nucleic acids.
Claims12
A purified and isolated DNA sequence encoding a mammalian early regulatory growth protein which comprises one or more histidine-cysteine finger amino acid sequences, said DNA capable of hybridizing with the mouse Egr-1 gene of FIGS. 1.1-1.8, under hybridization conditions which include 1 M NaCl and 10% dextran sulfate at 50.degree.-65.degree..
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