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US 5,206,150 AGranted
Composition of, method of producing and method of using a stabilized formulation for assaying peroxidase activity
AssigneeUniversity of Kentucky Research Foundation
InventorHsin-Hsiung Tai
Life of this patent
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Abstract
A formulation for use in detecting and/or determining peroxidase activity comprises a mixture in solution of tetramethylbenzidine, hydrogen peroxide, a buffering agent and bacitracin as a stabilizing agent. The formulation is used as a peroxidase substrate that is stable in solution for an extended period of time and provides enhanced color sensitivity.
Claims22
A formulation for the detection and determination of peroxidase activity, comprising:
- a chromatic compound;
- a peroxide;
- a water-miscible organic solvent;
- an aqueous buffer solution;
- and
- a stabilizing agent selected from a group consisting of bacitracin, bacitracin precursors, bacitracin components derived by hydrolysis and mixtures thereof.
3 more elements
A process for the preparation of a formulation for the detection and determination of peroxidase activity, comprising the steps of:
- forming a first solution by dissolving a chromatic compound in a water miscible organic solvent;
- forming a second solution by preparing an aqueous acidic buffer solution and adding in any order a stabilizing agent selected from a group consisting of bacitracin, bacitracin precursors, bacitracin components derived by hydrolysis and mixtures thereof and a peroxide;
- and
- mixing said first and second solutions to form said formulation.
1 more element
Dependencies are inferred from the claim text.