Method for assaying aliphatic alcohol, aliphatic aldehyde or .omega.-carboxylic acid derivatives thereof

A method for assaying aliphatic alcohol, aliphatic aldehyde or .omega.-carboxylic acid derivatives thereof, comprising reacting in an aqueous medium 1) a sample containing a compound of the formula, R'--CH.sub.2 OH or R'--CHO, wherein R'--CH.sub.2 OH is an aliphatic alcohol or its .omega.-carboxylic acid derivative, other than methanol and ethanol, and R'--CHO is a corresponding aliphatic aldehyde or its .omega.-carboxylic acid derivative, with 2) .omega.-carboxyalcohol oxidase having the following biochemical properties:

• enzymatic action:
• catalyzing both of the following reactions a) and b)
• a) R--CH.sub.2 OH+O.sub.2 .fwdarw.R--CHO+H.sub.2 O.sub.2
• b) R--CHO+O.sub.2 +H.sub.2 O.fwdarw.R--COOH+H.sub.2 O.sub.2
• wherein R--CH.sub.2 OH is an aliphatic alcohol or its .omega.,carboxylic acid derivative, other than methanol and ethanol, R--CHO is a corresponding aliphatic aldehyde or its .omega.-carboxylic acid derivative, and R--COOH is the oxidized form of R--CHO;
• substrate specificity:
• having substrate specificity on at least HO.sub.2 C--(CH.sub.2).sub.11 --OH, H.sub.3 C--(CH.sub.2).sub.11 --OH, H.sub.3 C--(CH.sub.2).sub.9 --OH, H.sub.3 C--(CH.sub.2).sub.7 --OH and H.sub.3 C--(CH.sub.2).sub.5 --OH, and having no substrate specificity on methanol, ethanol or glycerol;
• and
• utilization of coenzyme:
• does not require presence of NAD or NADP;
• and quantitatively determining an amount of a component consumed or generated in one of said reactions a) and b).