Okadaic acid testing for ciguatoxin
AssigneeHawaii Chemtect International
InventorDouglas L. Park
Life of this patent
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Abstract
A composition comprising okadaic acid for use as a positive control in assays for the detection of ciguatoxins and methods for making such compositions. In a preferred embodiment, the okadaic acid composition further comprises a carrier selected from the group consisting of fish extract, oils, oil/organic solvent mixtures, fatty-acid solutions, and non-ionic detergent solutions. A kit for detecting the presence of ciguatoxin or related polyether marine toxins in fish, comprising positive control supports impregnated with a composition comprising okadaic acid.
Claims33
A kit for detecting the presence of ciguatoxin or related polyether marine toxins in fish, comprising positive control reagents which react reproducibly with antibodies against ciguatoxins or related polyether marine toxins wherein the positive control reagents comprise:
- okadaic acid;
- and
- a carrier, selected from the group consisting of toxic fish extract, non-toxic fish extract, oils, oil/organic solvent mixtures, fatty-acid solutions, and non-ionic detergent solutions.
A method of preparing a positive control reagent for use in assays for the detection of ciguatoxins and related polyether marine toxins, comprising:
- mixing okadaic acid with a carrier, selected from the group consisting of toxic fish extract, non-toxic fish extract, oils, oil/organic solvent mixtures, fatty-acid solutions, and non-ionic detergent solutions wherein the okadaic acid/carrier mixture reacts reproducibly with antibodies against ciguatoxin or related polyether marine toxins;
- and
- applying the okadaic acid/carrier mixture to a support.
A method for performing a positive control assay in the detection of ciguatoxins and related polyether marine toxins comprising:
- mixing okadaic acid with a carrier;
- applying the okadaic acid/carrier mixture to a support;
- fixing the okadaic acid/carrier mixture to the support;
- contacting the okadaic acid/carrier mixture, bound to the support, to an antibody against ciguatoxin;
- washing any unbound antibody from the support;
- and
- quantitating the amount of antibody bound to the support.
A positive control reagent comprising:
- okadaic acid;
- and
- a carrier, selected from the group consisting of toxic fish extract, non-toxic fish extract, oils, oil/organic solvent mixtures, fatty-acid solutions, and non-ionic detergent solutions, wherein the okadaic acid/carrier mixture reacts reproducibly with antibodies against ciguatoxin or related polyether marine toxins.
Dependencies are inferred from the claim text.