Method for determining the presence or concentration of a bound enzyme
AssigneeBioControl Systems, Inc.
InventorsJr.; N. Robert Ward, Philip J. Lozier
Life of this patent
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Abstract
There is disclosed a process and a device for detecting and measuring (1) the amount of enzyme present as a detecting system following a nucleic acid hybridization reaction or immunoreaction; (2) the level and activity of free enzyme in a biological sample; (3) the level of enzyme from contaminating microorganisms present in a sample; and (4) enzymes from pure culture isolates for microbial identification and antimicrobial susceptibility testing.
Claims4
A method for determining the presence or concentration of an enzyme marker bound to an antibody, antigen or strand of nucleic acid generated in a hybridization assay or immunoassay, comprising the steps of:
- adding a substrate in a selected solution to a first column containing the enzyme marker further bound to a solid phase as a result of the hybridination assay or immunoassay, said substrate being reactive with said bound enzyme marker;
- incubating the first column to enzymatically convert said substrate to a product in an amount proportional to the amount of bound enzyme marker present;
- transferring the product and unreacted substrate onto a second column of no more than 500 theoretical plates, said second column containing sorbent selectively binding said product in the presence of said selected solution;
- eluting the product from the sorbent;
- and
- detecting the presence or concentration of the product to determine the presence or concentration of the bound enzyme marker.
Dependencies are inferred from the claim text.