Compositions and methods useful for genetic analysis
AssigneeLifecodes Corporation
InventorsNancy Y. Ip, Howard J. Baum
Life of this patent
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Abstract
A method for obtaining polynucleotide sequences which are useful for detecting Variable Tandem Repeat polymorphism at multiple genetic loci and other genetic analyses under high stringency conditions (hence, with high specificity) is herein disclosed. Also disclosed are polynucleotide sequences and other compositions useful for DNA polymorphism and other genetic analyses.
Claims7
A polynucleotide sequence which forms stable hybrids under high stringency conditions with genomic Variable Tandem Repeat DNA sequences at multiple, polymorphic loci of a human, wherein said high stringency conditions comprise an aqueous environment containing about 0.1 x SSC, or less, at about 65.degree. C., and wherein said polynucleotide sequence is selected from the group consisting of:
- (a) the human DNA insert in pAC329;
- (b) the human DNA insert in pAC344;
- (c) the human DNA insert in pAC365;
- and
- (d) mutational, insertional, or deletional variants of (a)-(d), wherein said variants form stable hybrids, under high stringency conditions, with DNA sequences which hybridize with said polynucleotide sequences of (a)-(d) above.
Dependencies are inferred from the claim text.