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US 5,206,135 AGranted
Use of the oxygenase activity of acetolactate synthase for herbicide detection
AssigneeE. I. Du Pont de Nemours and Company
InventorsLynn M. Abell, John V. Schloss
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Abstract
A process for the detection of herbicides that inhibit acetolactate synthase (EC 4.1.3.18) by use of the enzyme, a suitable substrate (pyruvate, .alpha.-aceto-lactate, .alpha.-ketobutyrate, or .alpha.-aceto-.alpha.-hydroxybutyrate), and an oxygen-sensitive electrode is disclosed.
Claims7
A process for detecting acetolactate synthase-inhibiting herbicides by the use of acetolactate synthase and an oxygen-sensitive electrode, which comprises
- mixing a solution to be tested with a substrate selected from the group consisting of pyruvate, .alpha.-ketobutyrate, .alpha.-acetolactate, and .alpha.-aceto-.alpha.-hydroxybutyrate;
- placing the mixture in contact with an oxygen-sensitive electrode;
- adding to the mixture an acetolactate synthase, in soluble form or immobilized to an inert support;
- adding to the mixture flavin adenine dinucleotide, thiamine pyrophosphate, a divalent metal, and a buffer to control the pH of the solution to the physiologic range of 6 to 10 pH;
- and
- monitoring the consumption of oxygen by the ensuing reaction by use of the oxygen-sensitive electrode.
3 more elements
Dependencies are inferred from the claim text.