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US 5,206,006 AGranted
Stabilized trinder reagent
AssigneeInstrumentation Laboratory Company
InventorsDario Frontini, Maurizio D'Alterio
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Abstract
Spontaneous aspecific coloration in so-called Trinder reagents which can alter subsequent colorimetric determinations is inhibited by adding compounds of the chelating agent class to the solution. The resultant compositions contain a peroxidase enzyme, a phenylpyrazone derivative, a compound of phenolic or aromatic amine structure and a stabilizer. Preferred stablizers are ethylenediaminetetraacetic acid (EDTA) and diethylenetriaminepentaacetic acid (DTPA).
Claims9
A composition of the Trinder type for detecting hydrogen peroxide evolved in a solution, consisting essentially of:
- a) a phenylpyrazone derivative,
- b) a phenol or an aromatic amine,
- c) a peroxidase enzyme,
- d) aqueous buffer, and
- e) a chelating agent comprising a polyalkylaminepolyacetic acid or a salt thereof.
2 more elements
Dependencies are inferred from the claim text.