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US 5,204,447 AGranted
Purification of factor XIII
AssigneeZymoGenetics, Inc.
InventorsPaul D. Bishop, Gerald W. Lasser
Life of this patent
Estimating the term…
Abstract
Methods for purifying factor XIII from a biological fluid are provided. The methods comprise precipitation of factor XIII by adjusting the pH of the biological fluid to 5.5 to 6.5 and recovering the precipitated factor XIII.
Claims19
A method for purifying factor XIII from a biological fluid, comprising:
- precipitating the factor XIII by adjusting the pH of the biological fluid to 5.5 to 6.5;
- and
- recovering the precipitated factor XIII.
A method for purifying factor XIII from a biological fluid, comprising:
- fractioning the biological fluid by anion exchange chromatography to produce an enriched fraction;
- precipitating the factor XIII by adjusting the pH of the enriched fraction to 5.5 to 6.5;
- and
- recovering the precipitated factor XIII.
1 more element
A method for purifying factor XIII from a biological fluid, comprising:
- adding to a biological fluid ammonium sulfate to about 30% to about 40% of saturation to produce a precipitate;
- resuspending the precipitate in a low ionic strength, mildly alkaline buffer;
- fractioning the resuspended precipitate by anion exchange chromatography to produce an enriched fraction;
- precipitating the factor XIII by adjusting the pH of the enriched fraction to 5.5 to 6.5 with piperazine buffer;
- recovering the precipitated factor XIII;
- dissolving the precipitated factor XIII to produce a solution;
- fractioning the solution by size exclusion chromatography to produce a second enriched fraction;
- and
- recovering the second enriched fraction.
6 more elements
Dependencies are inferred from the claim text.