US 5,204,262 A · Ethanol sensor for computerized fermentation control

Abstract

A probe for continuously measuring the precise concentration of liquid ethanol in a fermentation broth over the range of feasible fermentation temperatures. The probe includes a thermal sensor, a gas permeable membrane leading to a vapor channel, an ethanol vapor sensor in a vapor sensing chamber, and a microcomputer. The microcomputer is loaded with a first set of data defining the initial parameters of the fermentation broth and a set of data defining the ethanol diffusion rate through the gas permeable membrane as a function of the temperature of such fermentation broth. Inlet and outlet connection and tubular interconnections are provided for leading a flushing gas through the vapor channel, past the ethanol vapor sensor and out a vent outlet. Electrical lines leading from the thermal sensor to the microcomputer provide an input signal which is proportional to the temperature of the fermentation broth. Electrical lines also lead from the ethanol vapor sensor to the microcomputer to provide a signal which is proportional to the concentration of ethanol vapor. Evaluating software is loaded into the microcomputer to correlate the fermentation broth temperature, the ethanol vapor concentration and the ethanol diffusion rate through the gas permeable membrane as a function of temperature to provide an output of the actual concentration of liquid ethanol in the fermentation broth. On the basis of the ethanol liquid concentration so-determined, the microcomputer can automatically control temperature, pH, oxygen concentration and nutrient supply process parameters of the fermentation.

Claims13

Dependent claims

A probe for continuously measuring the precise concentration of liquid ethanol in a fermentation broth over the range of feasible fermentation temperatures, said probe comprising:

a. a thermal sensor;
b. a gas permeable membrane leading to a vapor channel;
c. an ethanol vapor sensor in a vapor sensing chamber;
d. a microcomputer, said microcomputer being loaded with a first set of data defining the initial concentration of fermentation broth, acid and cellulosic components and microbial concentration, and density, and a second set of data defining the ethanol diffusion rate through said gas permeable membrane as a function of the temperature of said fermentation broth;
e. inlet means for a flushing gas and outlet means for said flushing gas and tubular interconnections for said flushing gas, said tubular interconnections being provided for leading said flushing gas through said vapor channel, through said vapor sensing chamber, past said ethanol vapor sensor, and out through said outlet means;
f. electrical lines leading from said thermal sensor to said microcomputer, said electrical lines being constructed to carry an output signal which is proportional to the temperature of said fermentation broth;
g. electrical lines leading from said ethanol vapor sensor to said microcomputer, said electrical lines being constructed to carry an output signal which is proportional to the concentration of ethanol vapor;
and
h. evaluating software loaded into said microcomputer to correlate said output signal proportional to said fermentation broth temperature, said output signal proportional to said ethanol vapor concentration and said second set of data defining said ethanol diffusion rate through said gas permeable membrane as a function of temperature and to provide a single output consisting of the actual concentration of liquid ethanol in said fermentation broth.

6 more elements

A method for continuously measuring the precise concentration of liquid ethanol in a fermentation broth over the range of feasible fermentation temperatures, said method comprising the steps of:

a. contacting a fermentation broth with a thermal sensor, thereby continuously to measure the temperature of said fermentation broth, said thermal sensor thereby providing an electrical output signal which is proportional to the temperature of said fermentation broth;
b. contacting said fermentation broth with a gas permeable membrane, thereby to permit ethanol in said fermentation broth to diffuse, in the form of ethanol vapor, across said gas permeable membrane into an ethanol vapor zone;
c. flushing said ethanol vapor zone with a gas, said gas passing from a gas inlet zone into said ethanol vapor zone, then through a sensor zone in contact with an ethanol sensor, and from said sensor zone through an outlet zone to be vented;
d. determining the concentration of ethanol vapor in said sensor zone by means of said ethanol sensor, said ethanol sensor thereby providing an electrical output signal which is proportional to said concentration of ethanol vapor;
e. providing a microcomputer loaded with a first set of data defining the initial concentration of fermentation broth, acid and cellulosic component and microbial concentration, and density, and a second set of data defining the ethanol diffusion rate through said gas permeable membrane as a function of the temperature of such fermentation broth;
f. entering said electrical output signal proportional to said temperature of said fermentation broth, and entering said electrical output signal proportional to said concentration of ethanol vapor as inputs to said microcomputer;
and
g. correlating said data by means of evaluating software to correlate said fermentation broth temperature, said ethanol vapor concentration and said ethanol diffusion rate through said gas permeable membrane as a function of temperature to provide an output value consisting of the actual concentration of liquid ethanol in said fermentation broth.

5 more elements

Dependencies are inferred from the claim text.

Ethanol sensor for computerized fermentation control

Life of this patent

Ethanol sensor for computerized fermentation control

Life of this patent

Abstract

Claims13