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US 5,204,257 AGranted
Method of recovering bacteriophage
AssigneeAutoGen Instruments, Inc.
InventorsDavid A. DeBonville, Kelley A. Logan
Life of this patent
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Abstract
A method of recovering nucleic acid-containing particles from a liquid medium by contacting the liquid medium containing the particles with a mixture of hydroxylated silica beads and a salt solution to bind the nucleic acid-containing particles, centrifuging the mixture to pellet the bound particles, and separating the pellet from the liquid.
Claims17
A method of recovering bacteriophage from a liquid medium, comprising:
- contacting a liquid medium containing bacteriophage with a mixture of at least 0.5 molar inorganic chloride salt or acetic salt solution and a hydroxylated silica to bind the bacteriophage;
- centrifuging the resultant mixture to pellet the bound bacteriophage;
- and
- separating the pellet from the liquid.
1 more element
A method of recovering bacteriophage from liquid broth medium, comprising:
- mixing a liquid medium containing bacteriophage with a 0.5 to 2.0M inorganic chloride salt or acetic salt solution/hydroxylated silica mixture to bind the bacteriophage;
- incubating the mixture;
- centrifuging the incubated mixture to pellet the bound bacteriophage;
- and
- separating the pellet from the liquid.
2 more elements
Dependencies are inferred from the claim text.