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US 5,204,254 AGranted
Maltopentaose producing amylases
AssigneeConsortium fur Elektrochemische Industrie GmbH
InventorsGerhard Schmid, Anton Candussio, August Bock
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Abstract
A maltopentaose producing amylase, and its derivatives modified by gene manipulation, can be expressed in E. col. These amylases facilitate the production of maltopentaose.
Claims2
An isolated and purified maltopentaose producing A-180 amylase protein, which protein has the following properties:
- (a) a molecular weight of 180,000 daltons as measured by SDS polyacrylamide gel electrophoresis;
- (b) a pI of 4.65 as measured by isoelectric focusing;
- (c) is able to hydrolyze a starch solution to produce a product composition comprising greater than 80% maltopentaose;
- (d) is unable to hydrolyze .gamma.-cyclodextrin;
- (e) is thermostable up to 50.degree. C. and;
- (f) is stable within the pH range 5.5-11.0.
3 more elements
Dependencies are inferred from the claim text.