DNA isolation method
AssigneePioneer Hi-Bred International, Inc.
InventorAnil K. Jhingan
Life of this patent
Estimating the term…
Abstract
A novel method for the isolation of high molecular weight DNA from plants, yeast and bacteria using xanthate-forming compounds such as sodium/potassium ethyl xanthogenate is disclosed. The procedure does not require deproteination and yields clean DNA that is suitable for both PCR and Southern blotting. It can be utilized on a small scale without homogenizing the tissue. These features also facilitate automated screening of plant tissue samples, one of the labor-intensive techniques in molecular biology. This method is also adaptable for use in the field.
Claims7
A method for disrupting cell walls in a DNA extraction process, comprising the steps of contacting the cells with an aqueous solution comprising a compound or compounds that form xanthate reaction products with cell wall polysaccharides and isolating DNA from the cells.
Dependencies are inferred from the claim text.