Extraction of thymidine and other nucleosides
AssigneeE. I. Du Pont de Nemours and Company
InventorsJames A. Doncheck, James R. Millis, Paul E. Swanson
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Abstract
An enzymatic process for extracting a nucleoside, such as thymidine, from a biomass without substantial thymine production.
Claims8
An enzymatic process comprising grinding barley malt sprouts to finer than about 60 mesh, suspending the ground sprouts in water to a solids content of 3 to 15 weight percent, adding 1.0 to 10 mM magnesium salt, 1.0 to 10 mM calcium salt, 0.1 to 1 mM zinc salt, to the dispersion, holding the dispersion at 40.degree. to 60.degree. C. for 12 to 70 hours, and recovering at least one of thymidine, cytidine, uridine, guanosine or deoxy-guanosine from the product.
Dependencies are inferred from the claim text.