Recombinant poxvirus internal cores
AssigneeHealth Research, Inc.
InventorEnzo Paoletti
Life of this patent
Estimating the term…
Abstract
What is described is a modified recombinant poxvirus, such as vaccinia virus, fowlpox virus and canarypox virus, having an internal core containing DNA from a non-pox source in a nonessential region of the poxvirus genome. The recombinant poxvirus is modified by disassociating the internal core from outer membranes of the poxvirus. The DNA codes for and expresses a gene product in a cell cultured in vitro and in a host in vivo without productive replication of the virus in the cell or in the host. What is also described is a vaccine containing the modified recombinant poxvirus for inducing an immunological response in a host inoculated with the vaccine.
Claims17
A substantially purified recombinant poxvirus core devoid of viral envelope by in vitro modification, said core containing therein exogenous DNA in a nonessential region of the poxvirus genome;
- the core being capable of expression of the exogenous DNA when inoculated into a host, without production of infectious progeny.
An immunogenic composition for inducing an immunological response in a host inoculated with said composition, said composition comprising a carrier and a substantially purified recombinant poxvirus core devoid of viral envelope by in vitro modification, said core containing therein exogenous DNA in a nonessential region of the poxvirus genome;
- the core being capable of expression of the exogenous DNA when inoculated into the host, without production of infectious progeny.
A method for expressing a gene product in a cell cultured in vitro, which method comprises introducing into the cell a substantially purified recombinant poxvirus core devoid of viral envelope by in vitro modification, said core containing therein exogenous DNA in a nonessential region of the poxvirus genome;
- the core being capable of expression of the exogenous DNA when introduced into the cell, without production of infectious progeny.
A method for expressing a gene product in a host, which method comprises inoculating the host with a substantially purified recombinant poxvirus core devoid of viral envelope by in vitro modification, said core containing therein exogenous DNA in a nonessential region of the poxvirus genome;
- the core being capable of expression of the exogenous DNA when inoculated into the host, without production of infectious progeny.
Dependencies are inferred from the claim text.