Method and a kit containing means for the kinetic determination of factor XIII
AssigneeBehringwerke Aktiengesellschaft
InventorWerner Stuber
Life of this patent
Estimating the term…
Abstract
A method for the detection and for the determination of blood coagulation factor XIII in plasma, as well as a kit containing means which can be used in this method, are described. Used in this method are a fibrin-aggregation inhibitor, thrombin, a glutamine-containing peptide as substrate for factor XIII, an amine and a system for detecting ammonia which is formed. The kit containing means which can be used in this method contains a fibrin-aggregation inhibitor and a glutamine-containing peptide, where appropriate in addition to other components necessary for the method.
Claims12
A method for the determination of Factor XIII in a Factor XIII-containing sample in which the sample is activated with thrombin in the presence of a fibrin-aggregation inhibitor and incubated with a substrate for Factor XIII, with a primary amine and with a buffer substance in solution, and the ammonia which is formed is determined using NADH, glutamic dehydrogenase and ketoglutarate, wherein the substrate for Factor XIII is
- X-Gly-Pro-Gly-Gln-Ser-Lys-Val-Y-Gly-amide
- wherein X is selected from the group consisting of H, at least one naturally occurring amino acid, the D-form of at least one naturally occurring amino acid, and a dipeptide formed by any of these amino acids;
- and
- wherein Y is selected from the group consisting of Ile and Leu.
Dependencies are inferred from the claim text.