Process for the reduction of star activities
AssigneeBoehringer Mannheim GmbH
InventorsFrank Laue, Waltraud Ankenbauer, Gudrun Schmitz
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Abstract
The present invention provides a process for the reduction of non-specific star activities in the case of the specific cleavage of desoxyribonucleic acids by incubation with a restriction endonuclease in an appropriate buffer, wherein to the incubation batch is added an antibiotic which binds to the DNA on or near the star sequences of the enzyme but not within the specific recognition sequence of the restriction endonuclease.
Claims9
Process for the reduction of nonspecific star activities during the specific cleavage of deoxyribonucleic acids comprising incubation with a restriction endonuclease in an appropriate buffer, wherein an antibiotic which binds to the DNA on or near the star sequences of the enzyme but not within the specific recognition sequence of the restriction endonuclease is added to the incubation batch.
Dependencies are inferred from the claim text.