Monoclonal antibodies against hepatitis B virus
AssigneeNational Research Development Corporation
InventorsAlison H. Goodall, George Janossy, Howard C. Thomas
Life of this patent
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Abstract
The new hybridoma cell lines RF-HBs-1, RF-HBs-2 and RF-HBs-4 each secrete a nonoclonal antibody to hepatitis B surface antigen. The production of the antibodies may be carried out in vitro by culturing one of the cell lines or in vivo by establishing one of the cell lines as an ascites tumour in a mouse and isolating antibodies from the ascites fluid or from the serum. The antibodies have therapeutic, preventative and diagnostic uses in respect of hepatitis B virus infections and can be used to purify hepatitis B surface antigen. The relative specificities of the three monoclonal antibodies make them particularly useful in radiometric assay techniques employing specific combinations of the antibodies in solid phase.
Claims24
A monoclonal antibody to hepatitis B surface antigen which is secreted by hybridoma cell line RF-HBs-1.
A process of producing monoclonal antibodies to hepatitis B surface antigen which comprises culturing hybridoma cell line RF-HBs-1, and recovering the antibodies produced.
A method of diagnosing the presence of hepatitis B surface antigen in a biological sample, said method comprising the steps of:
- contacting said antigen with antibodies secreted by hybridoma cell line RF-HBs-1;
- and
- detecting the presence of said antigen by immunofluorescent microscopy or immuno-electron microscopy or in a solid-phase radiometric assay system or in an enzyme-linked immunoassay.
A method of isolating hepatitis B surface antigen from a biological sample which comprises contacting the biological sample with RF-HBs-1, antibody and optionally with RF-HBs-2 and/or RF-HBs-4 antibodies in the solid phase to cause hepatitis B surface antigen to being to the antibodies and subsequently separating the hepatitis B surface antigen material form the solid phase.
A kit comprising a fits container which contains a plastics substrate coated with either (1) RF-HBs-1 antibody and one or more other monoclonal antibodies which recognise hepatitis B surface antigen, or (2) with monoclonal antibodies to hepatitis B surface antigen secreted hybridoma cell lines RF-HBs-2 and RF-HBs-4, and a second container which contains antibody secreted by hybridoma cell line RF-HBs-1 to which antibody a radio-label or enzyme label has been attached.
Hybridoma cell line RF-HBs-1 which secretes a monoclonal antibody to hepatitis B surface antigen.
A kit comprising a first container which contains a plastics substrate coated with either (1) RF-HBs-1 antibody and one or more other monoclonal antibodies which recognise hepatitis B surface antigen, or (2) with a monoclonal antibody to hepatitis B surface antigen secreted by a hybridoma cell line selected form the group consisting of RF-HBs-2 and RF-HBs-4, and a second container which contains antibody secreted by hybridoma cell line RF-HBs-1 to which antibody a radio-label or enzyme label has been attached.
Dependencies are inferred from the claim text.