Cellulose binding fusion proteins having a substrate binding region of cellulase

A hybrid protein comprising a polypeptide and an amino acid sequence having a substrate binding region of a cellulase with the proviso that said substrate binding region is essentially lacking in cellulase activity, wherein said hybrid protein is obtained by a method comprising:

• (1) growing a transformed host cell containing an expression cassette comprising a DNA sequence encoding said hybrid protein whereby said hybrid protein is expressed;
• and
• (2) isolating said hybrid protein by binding said hybrid protein to a cellulose substrate of said cellulase.

A composition comprising:

• (1) a polysaccharide matrix comprising a cellulose substrate of a cellulase and
• (2) a hybrid protein wherein said hybrid protein comprises (a) a polypeptide and (b) an amino acid sequence having a substrate binding region of said cellulase with the proviso that said substrate binding region is essentially lacking in cellulase activity, wherein said polypeptide is fused to said substrate binding region and said substrate binding region binds to said cellulose substrate.

A composition comprising:

• (1) a cellulose matrix and
• (2) at least two different hybrid proteins wherein each of said proteins comprises (a) an amino acid sequence having a substrate binding region of a cellulase with the proviso that said substrate binding region is essentially lacking in cellulase activity said substrate binding region being fused to (b) a polypeptide having the activity of a polypeptide selected from the group consisting of a cellulase, a .beta.-glucosidase, an interleukin-2 and an alkaline phosphatase.

A composition comprising:

• (1) a cellulose and (2) a hybrid protein comprising (a) an amino acid sequence having substrate binding region of a cellulase with the proviso that said substrate binding region is essentially lacking in cellulase activity, said substrate binding region being fused to (b) a polypeptide having the activity of a polypeptide selected from the group consisting of an alkaline phosphatase, an interleukin 2, and a .beta.-glucosidase.